A Cytotoxic Compound from n-Hexane Fraction of Lantana camara Linn Leaves

Abstract

In this study, one triterpenoid compound from n-hexane fraction of Lantana camaraLinn leaves has cytotoxic activity was isolated. Isolation was carried out using gravity chromatographycolumnand purification by recrystallization method. Isolated compound was obtained white solid with melting point 252-253^oC. The structure of isolated compound was elucidated using spectroscopic analysis Ultraviolet (UV), Infrared (IR), ¹H-Nuclear Magnetic Resonance (¹H-NMR), ¹³C-Nuclear Magnetic Resonance (¹³C-NMR) Heteronuclear Multiple Bond Connectivity (HMBC),  Heteronuclear Multiple Quantum Correlation (HMQC), Distortionless Enhancement Polarization Transfer (DEPT) and comparative literature data, identified as Lantadene A (22β-angeloyloxy-3-oxoolean-12-en-28-oic-acid) with molecule formula C₃₅H₅₂O₅. The Lantadene A compound was evaluated for cytotoxic activity against shrimp larvae Artemia SalinaLeach using Brine Shrimp Lethality Test(BSLT) method, showed strong cytotoxic activity with an LC₅₀value of 48.97 µg/mL.