Flavon Compound from The Ethyl Acetate Extract of The Stem of Supit (Tetracera indica Merr.)

 PDF
Full Text
Muharni Muharni, Elfita Elfita, Riska Adillah, Heni Yohandini, Julinar Julinar

Abstract


Tetracera indica Merr. in Musi Banyuasin, is one of the traditional medicine used by the community for the treatment of kidney stone disease and gout, but this claim is not recorded in the treatment of kidney stones and gout in Indonesia.  In this study, isolation of antioxidant compound from ethyl acetate extracts of supit (Tetracera indica) was done. The isolation was carried out through step gradient polarity extraction, and separated and purified by chromatography technique. The determination of the structure of the isolated compound was performed by spectroscopy method including UV, IR, and NMR 1D and 2D, and antoxidant activity was determined  by DPPH method.  An  active antioxidant compound was isolated from ethyl acetate extract in form of yellow solid (15 mg).  Based on spectroscopic analysis the isolated compound was 5,8-dihydroxy-7-methoxyflavone.  The compound showed strong antioxidant activity (IC50 8.25 μg/mL) higher than standard ascorbic acid (IC50 11.3 μg/mL).  This data concluded that efficacy of supit (Tetracera indica) for the treatment related to antioxidant activity (uric acid) is proven by the identification of one antioxidant compound of this plant.

Keywords


Flavon , Ethyl acetate extract, Tetracera indica, antioxidant

References


Abdullah, F., Ismail, N.H., Jamaludin, F., Mashim, S.N.A.M., 2013, Xanthine Oxidase Inhibitory Activity of Tetracera Indica, Open Conf. Proc. J. 4, 93–94.

Ahmed, Q.U., Dogarai, B.B.S., Amiroudine, M.Z. a M., Taher, M., Latip, J., Umar, A., Muhammad, B.Y., 2012, Antidiabetic activity of the leaves of Tetracera indica Merr. (Dilleniaceae) invivo and invitro. J. Med. Plants Res., 6, 5912–5922.

Brewer, M.S., 2011, Natural Antioxidants: Sources, Compounds, Mechanisms of Action, and Potential Applications, Comprehensive Reviews in Food Science and Food Safety, 10(4),221-247

Chaudhary, A., Bhandari, A., and Pandurangan, A., 2012, Antioxidant potential and total phenolic content of methanolic bark extract of Madhuca indica (koenig) Gmelin, Anc Sci Life, 31(3):132-6

Dogarai, B.B.S., 2011. Phytochemical and antidiabetic activity investigations of tetracera. International Islamic University Malaysia.

Elfahmi, Herman, J., Woerdenbag, and OliverKayser,2014. Jamu: Indonesian traditional herbal medicine towards rational phytopharmacological use, Journal of Herbal medicine, 4(2), 51-73

Fitrya, Muharni, and , Maretha , K., 2012, Senyawa Fenolat dari Fraksi Etil Asetat Buah Tumbuhan Mempelas (Tetracera indica Merr.), Jurnal Penelitian Sains, 15(3),107-110

Hanafi,M., Nina A, Zorni Fadia, Nurbaiti, 2005, Indonesian Country Report,on Traditional medicine, BPPT, Indonesia

Heim, K. E., Tagliaferro, A. R., and Bobilya, D. J., 2002, Flavonoid Antioxidant: Chemistry, Metabolism and Structure-Activity Relationships. Journal of Nutritional Biochemistry, 13: 572-584.

Lima, C.C., Lemos, R.P.L., Conserva, L.M., 2014, Dilleniaceae family : an overview of its ethnomedicinal uses, biological and phytochemical profile. J. Pharmacogn. Phytochem., 3, 181–204.

Meyer, A. S., Heinonen, M., and Frankel, E. N,. 1998, Antioxidant Interactions of Catechin, Cyanidin, Caffeic Acid, Quercetin , and Elagic Acid on Human LDL Oxidation. Food Chemistry, 61 (1): 71-75.

Mariod A.A., Matthaus B., Hussein I.H. 2008, Antioxidant properties of methanolic extracts from different parts of Sclerocarya birrea. Int. J. Food Sci. Technol, 43:921–926

Muharni, Fitrya, Nurmaliana, R., 2016. Skrining fitokimia aktifitas antioksidan dan antibakteri dari tumbuhan obat tradisional etnis Musi. Palembang.

Muharni, Elfita, and Amanda, 2012, Biflavonoid from the stem bark of Gamboge (Garcinia xanthochymus), Indonesian Journal Chemistry , 11(2), 169-173

Md. Delowar, H., Md. Shahid, S., Syed Masudur, R. D., Md. Shohel, H., AFM Shahid-U.D and Mohammad, S.I., 2014, Investigation of total phenolic content and antioxidant activities of Azadirachta indica roots, Avicenna J. Phytomed, 4(2), 97-102.

Panthong, K., Pongcharoen, W., Phongpaichit, S., Taylor, W.C., 2006, Tetraoxygenated xanthones from the fruits of Garcinia cowa. Phytochemistry 67, 999–1004.

Selvi, A.T., Joseph, G., Jayaprakasha, G., 2003. Inhibition of growth and aflatoxin production in Aspergillus flavus by Garcinia indica extract and its antioxidant activity. Food Microbiol. 20, 455–460.

Tuanjai, N., Supalax, S., Thawatchai, Y., and Wittaya, N., 2011. New approach for evaluation of the antioxidant capacity based on scavenging DPPH free radical in micelle systems, Food Research International, 44(3), 798-806.

Yustian, I., Muharni, Sukarmi, S., Zulaicha, Arbi, M., 2012. Riset khusus eksplorasi pengetahuan lokal etnomedisin dan tumbuhan obat di Indonesia berbasis komunitas (Wilayah Musi II). Palembang.




DOI: http://dx.doi.org/10.20884/1.jm.2018.13.1.402

Metric logoArticle Metrics


This article has been viewed: 2689 (times)
PDF file viewed / downloaded: 913 (times)

Refbacks

  • There are currently no refbacks.


Copyright (c) 2018 Molekul

Logo Unsoed

Molekul

Jurnal Ilmiah Kimia
Department of Chemistry, Faculty of Mathematics and Natural Sciences,
Universitas Jenderal Soedirman, Purwokerto, Indonesia

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.