Gene Expression Changes and Anti-proliferative Effect of Noni (Morinda Citrifolia) Fruit Extract Analysed by Real Time-PCR

Full Text
Hermansyah Hermansyah, Susilawati Susilawati


To elucidate the anti-proliferative effect of noni (Morinda citrifolia) fruit extract for a Saccharomyces cerevisiae model organism, analysis of gene expression changes related to cell cycle associated with inhibition effect of noni fruit extract was carried out. Anti-proliferative of noni fruit extract was analyzed using gene expression changes of Saccharomyces cerevisiae (strains FY833 and BY4741).  Transcriptional analysis of genes that play a role in cell cycle was conducted by growing cells on YPDAde broth medium containing 1% (w/v) noni fruit extract, and then subjected using quantitative real-time polymerase chain reaction (RT-PCR).  Transcriptional level of genes CDC6 (Cell Division Cycle-6), CDC20 (Cell Division Cycle-20), FAR1 (Factor ARrest-1), FUS3 (FUSsion-3), SIC1 (Substrate/Subunit Inhibitor of Cyclin-dependent protein kinase-1), WHI5 (WHIskey-5), YOX1 (Yeast homeobOX-1) and YHP1 (Yeast Homeo-Protein-1) increased, oppositely genes expression of DBF4 (DumbBell Forming), MCM1 (Mini Chromosome Maintenance-1) and TAH11 (Topo-A Hypersensitive-11) decreased, while the expression level of genes CDC7 (Cell Division Cycle-7), MBP1 (MIul-box Binding Protein-1) and SWI6 (SWItching deficient-6) relatively unchanged. These results indicated that gene expression changes might associate with anti-proliferative effect from noni fruit extract. These gene expressions changes lead to the growth inhibition of S.cerevisiae cell because of cell cycle defect.


Morinda citrifolia, Noni fruit, Saccharomyces cerevisiae, anti-proliferative


Adames N.R., Schuk P.L., Chen, K.T., Murali, T.M., Tyson, J.j., Peccoud, J. (2015). Experimental testng of a new integrated model of the budding yeast START transition. Molecular Biology of the Cell. Vol 26, no. 22, 3966-3984.

Alexandrow, M.G. and Hamlin, J.L (2004). Cdc6 chromatin affinity is unaffected by Serine-54 phosphorylation, S-phase progression, and overexpression of cyclin A: Molecular Cellular Biology, 24(4); 1614-1627.

Borlando, L.R., and Mendez, J. (2008). CDC6; from DNA replication to cell cycle checkpoints oncogenesis: Carcinogenesis, 29(2), 237-43.

Calvert, M.E. and Lannigan, J. (2010). Yeast cellcycle analysis: combining DNA staining with cell and nuclear morphology: Current Protocols in Cytometry, 9(32), 1-6.

Calvert, M.E., Lanniga, j.A., and Pemberton, L.F. (2008). Optimization of yeast cell cycle analysis and morphological characterization by multispectral imaging flow cytometry: Cytometry, 73(9), 825-33.

Chan-Blanco, Y., Vaillant, F., Perez, A.M., and Brat, P. (2006). The noni fruit (Morinda citrifolia L.) : A review of agricultural research, nutritional and therapeutic properties. Journal of Food Composition Analysis, 19, 645-654.

Furusawa, E., Hirazumi, A., Story, S., and Jensen, J. (2003). Morinda citrifolia (Noni) on sarcoma 180 ascites tumour in mice. Phytotherapy Research, 17(10), 1158-64.

Gibson, U.E., Heid, C.A., and William, P.M. (1996). A novel method for real time quantitative RT-PCR: Genome Research. 6(10), 995-1001

Hermansyah, Sugiyama, P., Kaneko, Y. and Harashima, S. (2009). Yeast protein phosphatase Ptp2p and Msg5p are involved in G1-S transition, CLN2 transcription, and vacuole morphogenesis: Archive of Microbiology, 191, 721-733.

Lim, H.H., Goh, P-Y., Surana, U. (2015). Cdc20 is essential for the cyclosome-mediated proteolytis both Pds1 and Clb2 during M phase in budding yeast. Curent Biology, 8, 4, 231-234.

Potterat, O. And Hamburger, M. (2007). Morinda citrifolia (Noni) fruit-phytochemistry, pharmacology, safety, Planta Medica 73, 3, 191-199.

Siddiqui, B>S., Sattar, F.A, Ahmad, F., and Begum, S.. (2007). Isolation and structure elucidation of chemical constituents from the fruit of Morinda citrifolia Linn. Archives of Pharmacal Research, 30(8); 919-23

Takashima, J., Ikeda, Y., Komiyama, K., Hayashi, M., Kishida, A., and Ohsaki, A. (2007). New constituents from the leaves of Morinda citrifolia. Chemical and Pharmaceutical Bulletin , 55(20: 343-5,

Wang, M.Y., West, B.J., Jensen, C.J., Nowicki, D., Su, C., Palu, A.K., Anderson, G. (2002). Morinda citrifolia (Noni) : a literature review and recent advances in Noni research.

Acta Pharmacologika Sin; 23(12), 1127-41

Winston F., Dollard C. and Ricupero-Hovasse, S.L. (1995). Construction of a set of convenient Saccharomyces cerevisiae strains that are isogenic to S288C. Yeast 11, 53-55.


Metric logoArticle Metrics

This article has been viewed: 398 (times)
PDF file viewed / downloaded: 269 (times)


  • There are currently no refbacks.

Logo Unsoed


Jurnal Ilmiah Kimia
Department of Chemistry, Faculty of Mathematics and Natural Sciences,
Universitas Jenderal Soedirman, Purwokerto, Indonesia

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.