Gene Expression Changes and Anti-proliferative Effect of Noni (Morinda Citrifolia) Fruit Extract Analysed by Real Time-PCR |
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Abstract
To elucidate the anti-proliferative effect of noni (Morinda citrifolia) fruit extract for a Saccharomyces cerevisiae model organism, analysis of gene expression changes related to cell cycle associated with inhibition effect of noni fruit extract was carried out. Anti-proliferative of noni fruit extract was analyzed using gene expression changes of Saccharomyces cerevisiae (strains FY833 and BY4741). Transcriptional analysis of genes that play a role in cell cycle was conducted by growing cells on YPDAde broth medium containing 1% (w/v) noni fruit extract, and then subjected using quantitative real-time polymerase chain reaction (RT-PCR). Transcriptional level of genes CDC6 (Cell Division Cycle-6), CDC20 (Cell Division Cycle-20), FAR1 (Factor ARrest-1), FUS3 (FUSsion-3), SIC1 (Substrate/Subunit Inhibitor of Cyclin-dependent protein kinase-1), WHI5 (WHIskey-5), YOX1 (Yeast homeobOX-1) and YHP1 (Yeast Homeo-Protein-1) increased, oppositely genes expression of DBF4 (DumbBell Forming), MCM1 (Mini Chromosome Maintenance-1) and TAH11 (Topo-A Hypersensitive-11) decreased, while the expression level of genes CDC7 (Cell Division Cycle-7), MBP1 (MIul-box Binding Protein-1) and SWI6 (SWItching deficient-6) relatively unchanged. These results indicated that gene expression changes might associate with anti-proliferative effect from noni fruit extract. These gene expressions changes lead to the growth inhibition of S.cerevisiae cell because of cell cycle defect.
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References
Adames N.R., Schuk P.L., Chen, K.T., Murali, T.M., Tyson, J.j., Peccoud, J. (2015). Experimental testng of a new integrated model of the budding yeast START transition. Molecular Biology of the Cell. Vol 26, no. 22, 3966-3984.
Alexandrow, M.G. and Hamlin, J.L (2004). Cdc6 chromatin affinity is unaffected by Serine-54 phosphorylation, S-phase progression, and overexpression of cyclin A: Molecular Cellular Biology, 24(4); 1614-1627.
Borlando, L.R., and Mendez, J. (2008). CDC6; from DNA replication to cell cycle checkpoints oncogenesis: Carcinogenesis, 29(2), 237-43.
Calvert, M.E. and Lannigan, J. (2010). Yeast cellcycle analysis: combining DNA staining with cell and nuclear morphology: Current Protocols in Cytometry, 9(32), 1-6.
Calvert, M.E., Lanniga, j.A., and Pemberton, L.F. (2008). Optimization of yeast cell cycle analysis and morphological characterization by multispectral imaging flow cytometry: Cytometry, 73(9), 825-33.
Chan-Blanco, Y., Vaillant, F., Perez, A.M., and Brat, P. (2006). The noni fruit (Morinda citrifolia L.) : A review of agricultural research, nutritional and therapeutic properties. Journal of Food Composition Analysis, 19, 645-654.
Furusawa, E., Hirazumi, A., Story, S., and Jensen, J. (2003). Morinda citrifolia (Noni) on sarcoma 180 ascites tumour in mice. Phytotherapy Research, 17(10), 1158-64.
Gibson, U.E., Heid, C.A., and William, P.M. (1996). A novel method for real time quantitative RT-PCR: Genome Research. 6(10), 995-1001
Hermansyah, Sugiyama, P., Kaneko, Y. and Harashima, S. (2009). Yeast protein phosphatase Ptp2p and Msg5p are involved in G1-S transition, CLN2 transcription, and vacuole morphogenesis: Archive of Microbiology, 191, 721-733.
Lim, H.H., Goh, P-Y., Surana, U. (2015). Cdc20 is essential for the cyclosome-mediated proteolytis both Pds1 and Clb2 during M phase in budding yeast. Curent Biology, 8, 4, 231-234.
Potterat, O. And Hamburger, M. (2007). Morinda citrifolia (Noni) fruit-phytochemistry, pharmacology, safety, Planta Medica 73, 3, 191-199.
Siddiqui, B>S., Sattar, F.A, Ahmad, F., and Begum, S.. (2007). Isolation and structure elucidation of chemical constituents from the fruit of Morinda citrifolia Linn. Archives of Pharmacal Research, 30(8); 919-23
Takashima, J., Ikeda, Y., Komiyama, K., Hayashi, M., Kishida, A., and Ohsaki, A. (2007). New constituents from the leaves of Morinda citrifolia. Chemical and Pharmaceutical Bulletin , 55(20: 343-5,
Wang, M.Y., West, B.J., Jensen, C.J., Nowicki, D., Su, C., Palu, A.K., Anderson, G. (2002). Morinda citrifolia (Noni) : a literature review and recent advances in Noni research.
Acta Pharmacologika Sin; 23(12), 1127-41
Winston F., Dollard C. and Ricupero-Hovasse, S.L. (1995). Construction of a set of convenient Saccharomyces cerevisiae strains that are isogenic to S288C. Yeast 11, 53-55.
DOI: http://dx.doi.org/10.20884/1.jm.2017.12.1.333
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