RESISTANCE LEVEL OF Pseudomonas stutzeri AGAINST MERCURY AND ITS ABILITY IN PRODUCTION OF MERCURY REDUCTASE ENZYME

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Purkan Purkan, Safita Nurmalyya, Sofijan Hadi

Abstract


Mercury reductase is an enzyme that is able to reduce Hg2+ to Hg0 non toxic. This enzyme is usually produced by mercury resistant bacteria. The research wanted to determine the resistance of indigenous Pseudomonas stutzeri isolate toward mercury and to explore the mercury reductase activity which is produced by the bacteria. The results of resistance assay of the Pseudomonas stutzeri toward mercury ion showed that the isolate could survive in media containing HgCl2 up to a concentration of 80 µM. The bacteria could produce mercury reductase optimally at the 24th of fermentation time. The enzyme showed optimum activity at pH 7 and temperature of 45 oC

Keywords


Pseudomonas stutzeri, mercury reductase, mercury

References


Barkay, T., Miller, S. M., & Summers, A. O. (2003). Bacterial mercury resistance from atoms to ecosystems. FEMS microbiology reviews, 27(2-3), 355-384.

https://doi.org/10.1016/S0168-6445(03)00046-9

Brown, N. L., Shih, Y. C., Leang, C., Glendinning, K. J., Hobman, J. L., & Wilson, J. R. (2002). Mercury transport and resistance. Biochemical Society Transactions, 30(4), 715-718.

https://doi.org/10.1042/bst0300715

Dash, H. R., & Das, S. (2012). Mercury resistant marine bacterial population from Bhitarkanika mangrove ecosystem, Odisha. In Proceedings of National Conference on Mangrove Wetlands and Near Shore Marine Ecosystems from Sustainability Issues to Management and Restoration. School of Environmental Sciences, Jawaharlal Nehru University, New Delhi (pp. 48-49).

Giovanella, P., Cabral, L., Bento, F. M., Gianello, C., & Camargo, F. A. O. (2016). Mercury (II) removal by resistant bacterial isolates and mercuric (II) reductase activity in a new strain of Pseudomonas sp. B50A. New biotechnology, 33(1), 216-223.

https://doi.org/10.1016/j.nbt.2015.05.006

Kannan, S. K., & Krishnamoorthy, R. (2006). Isolation of mercury resistant bacteria and influence of abiotic factors on bioavailability of mercury—a case study in Pulicat Lake North of Chennai, South East India. Science of the Total Environment, 367(1), 341-353.

https://doi.org/10.1016/j.scitotenv.2005.12.003

Lehninger, A.L. (2010), Dasar Dasar Biokimia, Jilid 1, Penerjemah Maggy Thenawidjaya, Penerbit Erlangga, Jakarta, 159-160, 235-250.

Meryandini, A., Widosari, W., Maranatha, B., Sunarti, T. C., Rachmania, N., & Satria, H. (2009). Isolasi bakteri selulolitik dan karakterisasi enzimnya. Makara Sains, 13(1), 33-38.

Olson, G. J., Porter, F. D., Rubinstein, J., & Silver, S. (1982). Mercuric reductase enzyme from a mercury-volatilizing strain of Thiobacillus ferrooxidans. Journal of bacteriology, 151(3), 1230-1236.

Pelczar, M.J., and Chan, E.C.S. (2010), Dasar – Dasar Mikrobiologi, Jilid 1, Penerjemah: Hadioetomo, R.S., Penerbit UI Press, Jakarta, 132-142, 326-327

Robinson, J. B., & Tuovinen, O. H. (1984). Mechanisms of microbial resistance and detoxification of mercury and organomercury compounds: physiological, biochemical, and genetic analyses. Microbiological reviews, 48(2), 95-124.

Takeuchi, F., & Sugio, T. (2005). Volatilization and recovery of mercury from mercury-polluted soils and wastewaters using mercury-resistant Acidithiobacillus ferrooxidans strains SUG 2-2 and MON-1. Environmental sciences: an international journal of environmental physiology and toxicology, 13(6), 305-316.

Zeroual, Y., Moutaouakkil, A., Dzairi, F. Z., Talbi, M., Chung, P. U., Lee, K., & Blaghen, M. (2003). Purification and characterization of cytosolic mercuric reductase from Klebsiella pneumoniae. Annals of microbiology, 53(2), 149-160.




DOI: http://dx.doi.org/10.20884/1.jm.2016.11.2.256

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Jurnal Ilmiah Kimia
Department of Chemistry, Faculty of Mathematics and Natural Sciences,
Universitas Jenderal Soedirman, Purwokerto, Indonesia

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