Hemostatic Effect of Ethanol Extract of Piper betle, Linn Leaves to Male Mice

Sadakata Sinulingga, Subandrate Subandrate, Bebbi Arisya Kesumaputri, Galuh Anggraini


Hemorrhage occurs in most of the dental care. Untreated hemorrhage could cause excessive blood loss, hypotension, and cyanosis. A Natural resource that reported has an hemostatic effect is ethanol extract of betel leaves (Piper betel, Linn).The aim of this study is to find the minimum concentration of ethanol extract of betel leaves which capable of shortening the bleeding time in mice. The experimental study used pretest-posttest with control group design was conducted on 35 mice that divided into 7 group which are negative control, positive control (feracrylum 1%), the ethanol extract of betel leaves 1%, 5%, 10%, 15%, and 20%. All mice were injected heparin intravenously. Miceâs tail was cut at diameter 3 mm and pretest bleeding time was counted. Miceâs tail was recut at diameter 4 mm, given treatment for 5 seconds and posttest bleeding time was counted. Results of paired t-test showed that reduction of bleeding time between pretest and posttest was significant (p<0,050). The enhancement of ethanol extract of betel leaves concentration leads to better hemostatic effect. Results of ANOVA test showed that comparison of posttest bleeding time among groups was significant (p<0,050). The minimum concentration of ethanol extract of betel leaves which capable of shortening the bleeding time in mice is 5%.


bleeding, bleeding time, dental care, the ethanol extract of betel leaves.

Full Text:

View Counter: Abstract = 123 (times), and PDF = 82 (times)


Ashok, P. K., dan Upadhyaya, K. (2012) Tannins are astrigent. Journal of Pharmacognosy and Phytochemistry, 1(3), 45-50.

Barbosa, E. A. (2014). An overview On The Biological and Pharmacological Activities of Saponins. International Journal of Pharmacy and Pharmaceutical Sciences, 6(8), 47-50.

Bele, A. A., Jadhav, V. M., Kadam, V.J. (2010). Potential of Tannins: A Review. Asian Journal of Plant Sciences, 9(4), 209-214.

Bhalerao, S. A., Verma, D. R., Gavankar, R. V., Teli, N. C., Rane, Y. Y., Didwana, V. S., et al. (2013). Research and Review: Phytochemistry, Pharmacological Profile and Therapeutic Uses of Piper Betle, Linn. Journal of Pharmacognosy and Phytochemistry, 1(5), 12-18.

Bissa, S, Songara, D, dan Bohra, A. (2007). Traditions in oral hygiene: chewing of betel (Piper betle, Linn.) leaves. Current Science, 92(1), 26-8.

Borle, R. M. (2014). Textbook of oral and maxillofacial surgery. New Delhi: Jaypee Brothers Medical Publishers.

Chakraborty, B. S. (2011). Antimicrobial, antioxidative and antihemolytic activity of Piper betel leaf extracts. International Journal of Pharmacy and Pharmaceutical Sciences, 3(3), 192â199.

Chandra, V., Tripathi, S., Verma, N.K., Singh, D. P., Chaudhary, S. K., dan Roshan, A. (2011). Piper betle: phytochemistry, traditional use and pharmacological activity â a review. Chinese Journal of Integrative Medicine, 4(4), 216-223.

Grace, A. S. (2010). The hemostatic effect of A. Vulgaris extract in traumatic bleeding on Mus Musculus. Asia Pacific Dental Students Journal, 1(2), 174-183.

Gray, E., Hogwood, J., dan Mulloy, B. (2012). The anticoagulant and antithrombotic mechanisms of heparin. Handbook of Experimental Pharmacology, 207, 43-61

Greene, T. K., Schiviz, A., Hoellriegl, W., Poncz, M., dan Muchitsch, E. M. (2010). Towards a standardization of the murine tail bleeding model. Journal of Thrombosis and Haemostasis, 8(1), 2820-2802.

Lahoti, K., Aggarwal, G., Shashi, S., dan Laddha, A. (2010). Hemostasis during hypospadias surgery via topical application of feracrylum citrate: a randomized prospective study. Journal of Indian Associatin of Pediatric Surgeons, 15(3), 87-89.

Mohan, M., Gupta, A., dan Shenoy, V. (2011). Pharmacological agents in dentistry: a review. British Journal of Pharmaceutical Research. 5(3), 66-87.

Nirmala, B. R, dan Kumari, S. O. (2015). Phytochemical analysis of Piper Betel leaf extract. World Journal of Pharmacy and Pharmaceutical Sciences, 4(1), 699-703.

Periyanayagam, K., Jagadeesan, M., Kavimani, S., dan Vetriselvan. (2012) Pharmacognostical and physicochemical profile of the leaves of Piper betle, Linn â valuable assessment of itâs quality. Asian Pacific Journal of Tropical Biomedicine, 2(5), 31-39.

Pradhan, D., Suri, K. A., dan Biswasroy, P. (2013). Golden heart of the nature: Piper betle L. Journal of Pharmacognosy and Phytochemistry, 1(6),147-167.

Rupa, S., Banik, dan Jayanta, K. (2013). A review on Piper betle leaf. International Journal of Pharmaceutical Sciences and Research, 4(12, 45-49.

Sam, S. (2008). Hemorrhagic complications of anticoagulant and thrombolytic treatment. Chest, 13(3), 133-147.

Singh, K. (2016). Role of local instillation of one percent feracrylum and haemocogulase on wound healing. International Jornal of Research in Medical Science, 4(1), 169-176.

Tedjasulaksana, R. (2013). Ekstrak etil asetat dan etanol daun sirih (Piper betle, L) dapat memperpendek waktu perdarahan mencit (Mus musculus). Jurnal Kesehatan Gigi, 1(1), 32-39.

DOI: http://dx.doi.org/10.20884/1.jm.2017.12.1.264


  • There are currently no refbacks.

Creative Commons License
Jurnal Ilmiah Kimia Molekul by Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Jenderal Soedirman, Purwokerto, Indonesia is licensed under a Creative Commons Attribution 4.0 International License.
Based on a work at jmolekul.com.
Permissions beyond the scope of this license may be available at http://jmolekul.com/.